Regulatory

Part:BBa_K5486001

Designed by: Julian Borbeck   Group: iGEM24_Tuebingen   (2024-10-01)


NarL-LuxR fusion regulator

Fusion protein of signal reciever domain of NarL from Escherichia Coli combined with DNA binding domain LuxR from Aliivibrio fischeri. The fusion protein was designed using ClusterControl.

This part improves on BBa_C0062 by making it possible to control the output of the DNA binding domain using a different input

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 552
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 552
    Illegal NotI site found at 239
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 342
    Illegal BamHI site found at 153
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 552
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 552
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None